Human Dkk-1 ELISA Kit
SIGMA/RAB0143 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Dkk-1 ELISA Kit, Cat. No. RAB0143. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Dkk-1 ELISA Kit, Cat. No. RAB0143, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 100 pg/mL standard curve range: 122.9-30000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type urine sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Evidence strongly indicates that Dkk-1 protein functions as a potent inhibitor of the Wnt signaling pathway to enable appropriate positioning and development of the embryonic brain and other organ structures. hDkk-1 protein also has a pro-apoptotic function. |
| General description: | The human Dkk-1 (hDkk-1) gene, a transcriptional target of the p53 tumor suppressor, encodes a powerful inhibitor of the Wnt signaling pathway and regulates the spatial patterning/morphogenesis of the mammalian central nervous system.The Dkk-1 gene, also called Dickkopf-1 , is a prototype member of a recently identified gene family that encodes secreted glycoproteins that control cell fate and neural patterning during embryonic development. This gene is located on human chromosome 10q. |
| Immunogen: | Recombinant Human DKK1 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 5X Assay/Sample Diluent Buffer D (Item K); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |