Human EGF ELISA Kit
SIGMA/RAB0149 - for serum, plasma, cell culture supernatant.
Synonym: EGF ELISA Kit
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human EGF ELISA Kit, Cat. No. RAB0149. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human EGF ELISA Kit, Cat. No. RAB0149, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 0.82-200 pg/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type plasma sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Human EGF ELISA Kit has been used to examine the concentration of epidermal growth factor (EGF) in adipose derived stem cell conditioned medium (ADSC-CM). |
| Biochem/physiol Actions: | Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, which binds to EGF receptor. EGF increases proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It blocks gastric acid and pepsin secretion and enhances gastrointestinal cell proliferation. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is associated with hypomagnesemia.. |
| General description: | The Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EGF in serum, plasma and cell culture supernatants. |
| Immunogen: | Recombinant Human EGF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |