Human ErbB2 / HER2 ELISA Kit
SIGMA/RAB0173 - for serum, plasma, cell culture supernatant and urine
Synonym: ERBB2; HER2; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human ErbB2 / HER2 ELISA Kit, Cat. No. RAB0173. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human ErbB2 / HER2 ELISA Kit, Cat. No. RAB0173, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 8.19-2000 pg/mL |
| detection method | colorimetric |
| input | sample type urine sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | ERBB2 (erb-b2 receptor tyrosine kinase 2) is considered as a therapeutic target in breast cancer. It controls the concentration of intracellular calcium in SKBR3 (breast cancer cell line) cells. |
| General description: | ERBB2 (erb-b2 receptor tyrosine kinase 2) is a tyrosine kinase receptor that belongs to the ErbB family. It is also known as human epidermal growth factor receptor 2 (HER2)/Neu and is located on human chromosome 17q12. The human ErbB2 (epidermal factor growth factor receptor 2) ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB2 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human ErbB2 / HER2 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |