Human Fas Ligand ELISA Kit
SIGMA/RAB0178 - for serum, plasma, and cell culture supernatants
Synonym: FasL
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Fas Ligand ELISA Kit, Cat. No. RAB0178. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Fas Ligand ELISA Kit, Cat. No. RAB0178, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.37-1000 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS). |
| General description: | The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human Fas Ligand |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |