Human bFGF ELISA Kit
SIGMA/RAB0182 - for serum, plasma, cell culture supernatant and urine
Synonym: Basic fibroblast growth factor; FGF-2; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2; bFGF
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human bFGF ELISA Kit, Cat. No. RAB0182. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human bFGF ELISA Kit, Cat. No. RAB0182, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 50 pg/mL standard curve range: 102.4-10000 pg/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type serum sample type plasma sample type urine |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | Human bFGF ELISA kit has been used: • to quantify the level of residual cytokines in decellularized meningeal scaffolds (DMS) • to measure cytokine levels in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (RMS/CMS) • to analyze basic-fibroblast growth factor (bFGF) concentration in cell culture medium |
| Biochem/physiol Actions: | FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells, including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. MicroRNA (miR)-503 targets FGF2 mRNA and inhibits migration and invasiveness of osteosarcoma (OS) cells. |
| General description: | The Human bFGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human bFGF in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human basic-FGF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B); 20X Wash Buffer (Item B) |