Mouse bFGF ELISA Kit
SIGMA/RAB0184 - for serum, plasma and cell culture supernatant
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse bFGF ELISA Kit, Cat. No. RAB0184. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse bFGF ELISA Kit, Cat. No. RAB0184, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 18.8-600 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Mouse bFGF ELISA Kit has been used for evaluating the concentration of fibroblast growth factor (basic) in samples by ELISA. |
| Biochem/physiol Actions: | Fibroblast growth factor (basic) (bFGF), also known as fibroblast growth factor 2 induces neuronal proliferation, neurite outgrowth, chemotaxis, cell adhesion and migration. bFGF derived from airway smooth muscle cells plays a key role in the formation of lymphocyte-derived membrane conduits for intracellular communications. FGF2 basically functions in the formation of new blood vessels and promotes angiogenesis in cancer cells and chronic inflammatory diseases. FGF2 promotes prostate cancer by causing genome instability. |
| General description: | The Mouse bFGF (Fibroblast Growth Factor basic) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse bFGF in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Mouse basic-FGF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |