Human GDNF ELISA Kit
SIGMA/RAB0205 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human GDNFÂ ELISA Kit, Cat. No. RAB0205. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human GDNFÂ ELISA Kit, Cat. No. RAB0205, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 4 pg/mL standard curve range: 2.74-2000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type urine sample type cell culture supernatant(s) sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Glial cell derived neurotrophic factor (GDNF) is a neurotrophic factor involved in dopaminergic and neurodevelopment. It plays a vital role in self-renewal of stem cells. In addition, GDNF also facilitates differentiation of chained undifferentiated spermatogonia. GDNF with its receptors, activates various signaling pathways essential for maintaining cellular homeostasis. Mutated gene is associated with the development of schizophrenia. GDNF is a potential target for treating Parkinson′s disease. |
| General description: | Glial cell derived neurotrophic factor (GDNF) is encoded by the gene mapped to human chromosome 5p14.1−q13.3. It belongs to the transforming growth factor β (TGF-β) superfamily. GDNF is abundantly found in gliomas. |
| Immunogen: | Recombinant Human GDNF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |