Human sICAM1 ELISA Kit
SIGMA/RAB0219 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human sICAM1 ELISA Kit, Cat. No. RAB0219. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human sICAM1 ELISA Kit, Cat. No. RAB0219, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 150 pg/mL standard curve range: 0.313-20 ng/mL |
| detection method | colorimetric |
| input | sample type urine sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | For research use only. Not for use in diagnostic procedures. Human sICAM1 (intercellular adhesion molecule 1) ELISA Kit has been used for measuring sICAM1 levels in the endothelial cell-conditioned medium. |
| Biochem/physiol Actions: | The expression of ICAM1 (intercellular adhesion molecule 1) is strongly increased by Interleukin-1 (IL-1) and interferon (IFN)-γ. It is expressed on lymphoid cells where it is required for phorbol ester-induced aggregation. It participates in LFA-1-dependent NK cell stimulation through its attachment to actin cytoskeleton through α-actinin or ezrin. It also acts as the receptor for rhinovirus, a common cold virus, and Plasmodium falciparum. Soluble ICAM-1 (sICAM1) levels are elevated in pregnant women with gestational diabetes mellitus (GDM), which is an outcome of endothelial cell activation and dysfunction. In addition, ICAM-1 also has an essential role in binding circulating leukocytes to the blood vessel wall and trans-endothelial migration to vascular intima. Meta-analysis of case-control studies show that rs1799969 polymorphism in ICAM-1 gene is linked with increased susceptibility to cancer. sICAM1 might act as a biomarker for unipolar and bipolar patients. |
| General description: | The Human sICAM-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human soluble ICAM-1 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human ICAM1 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |