Mouse ICAM-1 ELISA Kit
SIGMA/RAB0220 - for serum, plasma and cell culture supernatant
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse ICAM-1 ELISA Kit, Cat. No. RAB0220. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse ICAM-1 ELISA Kit, Cat. No. RAB0220, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 20 pg/mL standard curve range: 24.69-6000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Intercellular adhesion molecule-1 (ICAM-1) is a member of immunoglobulin (Ig) superfamily. It is an inducible transmembrane glycoprotein. ICAM-1 mediates adhesion of circulating leukocytes to the endothelial membrane and blood vessel. ICAM-1 might cause atherosclerosis and the polymorphism in ICAM-1 has reduced risk to cardiovascular diseases. IL-6 induces the upregulation of ICAM-1 and leads to metastasis of osteosarcoma cells. ICAM-1 causes invasion and metastasis of breast cancer cells. ICAM-1 is an important receptor for rhinovirus and Haemophilus influenzae binding. Upregulation of ICAM-1 in the respiratory tract of chronic airway limitation patients increases the risk of respiratory infections. |
| General description: | The Mouse ICAM-1 (Intercellular Adhesion Molecule-1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of mouse ICAM-1 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Mouse ICAM1 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 5X Assay/Sample Diluent Buffer D (Item K); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |