Human IFN γ ELISA Kit
SIGMA/RAB0222 - for serum, plasma, cell culture supernatant and urine
Synonym: IFN-gamma; Interferon gamma
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human IFN γ ELISA Kit, Cat. No. RAB0222. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human IFN γ ELISA Kit, Cat. No. RAB0222, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 20.6-15000 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type urine sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA)Human IFN γ ELISA Kit has been used for determining interferon-gamma levels in cell culture supernatant and macrophages. |
| Biochem/physiol Actions: | IFNG (interferon-γ) is a type-2 interferon, which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis. |
| General description: | The Human IFN- ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN- in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human IFN-γ |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |