Mouse IL-10 ELISA Kit
SIGMA/RAB0245 - for serum, plasma and cell culture supernatant
Synonym: IL-10; Interleukin-10
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse IL-10 ELISA Kit, Cat. No. RAB0245. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse IL-10 ELISA Kit, Cat. No. RAB0245, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 45 pg/mL standard curve range: 78.13-5000 pg/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type serum sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | IL-10 (interleukin 10) is an anti-inflammatory cytokine. In mouse, IL-10 produced by the CD4+ Th2 (T helper cell 2) subset, inhibits the expression of cytokines specifically IFN-3 (interferon), by Thl (T helper cell 1) clones. mIL-10 has pleiotropic property and it interacts with various cell types such as thymocytes, cytotoxic T cells, mast cells, B cells, and macrophages. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. B- cell specific IL-10 might have a suppressive role in systemic lupus erythematosus (SLE). |
| General description: | The Mouse IL-10 (Interleukin-10) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Mouse IL-10 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 5X Assay/Sample Diluent Buffer D (Item K); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |