Human IL-12 p70 ELISA Kit
SIGMA/RAB0252 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human IL-12 p70 ELISA Kit, Cat. No. RAB0252. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human IL-12 p70 ELISA Kit, Cat. No. RAB0252, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 0.8-600 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type urine sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Human IL-12 p70 ELISA kit has been used to determine the concentrations of IL-12, IL-10, IL-1β and TNFα in cell culture supernatants. |
| Biochem/physiol Actions: | IL-12A (interleukin 12 A) is essential for differentiation of Th1 (type 1) cells. Polymorphisms in IL-12A gene is associated with Graves’ and Alzheimer’s disease, rheumatoid arthritis and asthma. |
| General description: | The Human IL-12(P70) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12(P70) in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human IL-12 p70 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |