Mouse IL-13 ELISA Kit
SIGMA/RAB0257 - for serum, plasma and cell culture supernatant
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse IL-13 ELISA Kit, Cat. No. RAB0257. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse IL-13 ELISA Kit, Cat. No. RAB0257, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 6 pg/mL standard curve range: 8023-2000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type cell culture supernatant(s) sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Mouse IL-13 ELISA Kit has been used to measure the concentration of interleukin (IL)-13 in serological sampleand bronchoalveolar lavage fluids (BALFs). |
| Biochem/physiol Actions: | Interleukin 13 (IL13) is up-regulated during parasitic infections and participates in host′s protective immunity against Schistosoma japonicum. This protein promotes eosinophil activation resulting in elevated specific IgE against adult and larval antigens. During acute infection it promotes egg expulsion through increased intestinal contractility. It acts as a liver protective agent where it promotes the formation of liver granulomas in a CD4+ T cell-mediated manner, which results in liver fibrosis. In airway disorders, such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis lung disease, IL13 promotes the formation of airway goblet cells, resulting in excess mucus secretion, which in turn, is regulated by IL13-activated autophagy. |
| General description: | The Mouse IL-13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-13 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Mouse IL-13 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |