Human IL-1 β ELISA Kit
SIGMA/RAB0273 - for serum, plasma, cell culture supernatant and urine
Synonym: Il-1 beta; Interleukin-1 beta
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human IL-1 β ELISA Kit, Cat. No. RAB0273. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human IL-1 β ELISA Kit, Cat. No. RAB0273, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 0.3 pg/mL standard curve range: 0.48-100 pg/mL |
| detection method | colorimetric |
| input | sample type urine sample type serum sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Human IL-1 β ELISA kit has been used to measure the concentration of interleukin 1β (IL-1 β) in cell culture supernatants. |
| Biochem/physiol Actions: | Interleukin 1β (IL-1 β) is a proinflammatory cytokine, which is implicated in induction of various acute and chronic inflammation. Hence, IL1B can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling. IL-1β protein increases the production of endogenous 92kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1). Elevated concentration of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint. IL-1β brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura (HSP). |
| General description: | The Human IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 β in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human IL-1β |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |