Rat IL-1 β ELISA Kit
SIGMA/RAB0278 - for cell and tissue lysates
Synonym: Il-1 beta; Interleukin-1 beta
Product Type: Chemical
Standard Curve : Typical Standard Curve for Rat IL-1 β ELISA Kit, Cat. No. RAB0278. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Rat IL-1 β ELISA Kit, Cat. No. RAB0278, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 80 pg/mL standard curve range: 68.59-50000 pg/mL |
| detection method | colorimetric |
| input | sample type cell lysate sample type tissue lysate |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | rat |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures Rat IL-1 Β ELISA Kit has been used to measure IL-1 Β levels . Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Pro-inflammatory cytokine IL-1 Β (IL1B), which is highly expressed on Β-adrenoreceptor in obese adipose tissues, is implicated in induction of uncoupling protein 1 (UCP1) expression via extracellular signal-regulated kinase (ERK) activation in adipocytes. Macrophage autophagy inhibits the production of inflammasome-dependent IL-1Β and thereby it plays a vital role in reducing acute toxin-induced liver injury and death. In rats, IL-1Β enhanced neurogenesis and plasticity of carotid bodies (CB) by triggering ERK1/2 activation. IL-1Β plays a vital role in alteration of glomerular hemodynamics via stimulating the production of prostaglandin E2 (PGE2) in the mesangial cells. |
| General description: | The Rat IL-1 β ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1β cell lysate and tissue lysate. |
| Immunogen: | Recombinant Rat IL-1 β |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); 2X Cell Lysis Buffer (Item J); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |