Human IL-1ra ELISA Kit
SIGMA/RAB0283 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human IL-1RA ELISA Kit, Cat. No. RAB0283. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human IL-1RA ELISA Kit, Cat. No. RAB0283, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 100 pg/mL standard curve range: 0.124-30 ng/mL |
| detection method | colorimetric |
| input | sample type serum sample type cell culture supernatant(s) sample type plasma sample type urine |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α and IL-1β by competitively binding to their type I and type II receptors. IL-1RA plays a vital role in inhibition of acute pro-inflammatory response and forbidding chronic inflammation from damaging healthy tissues. Abnormal expression of IL-1RA leads to development of inflammatory or autoimmune related diseases. Allelic polymorphism of IL-1RA leads to inflammatory bowel disease (IBD). Therapeutically, IL-1RA may help in the treatment of sepsis, rheumatoid arthritis, asthma. Overexpression of IL-1RA is observed in serum of psoriatic arthritis (PsA) patients. |
| General description: | The Human IL-1ra ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1ra in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human IL-1 Receptor Antagonist |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |