Human IL-2 ELISA Kit
SIGMA/RAB0286 - for serum, plasma, cell culture supernatant and urine
Synonym: IL-1 beta; Interleukin-1 beta
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human IL-2 ELISA Kit, Cat. No. RAB0286. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human IL-2 ELISA Kit, Cat. No. RAB0286, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 4 pg/mL standard curve range: 2.07-1500 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type cell culture supernatant(s) sample type urine sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | Human IL-2 ELISA Kit has been used in the analysis of interleukins levels in human plasma. |
| Biochem/physiol Actions: | IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer, asthma and Mycoplasma pneumoniae infection in children. Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways. |
| General description: | The Human IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-2 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human IL-2 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |