Mouse IL-4 ELISA Kit
SIGMA/RAB0300 - for cell and tissue lysates
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse IL-4 ELISA Kit, Cat. No. RAB0300. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse IL-4 ELISA Kit, Cat. No. RAB0300, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1 pg/mL standard curve range: 0.819-200 pg/mL |
| detection method | colorimetric |
| input | sample type cell lysate sample type tissue lysate |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | For research use only. Not for use in diagnostic procedures. Mouse IL-4 ELISA kit has been used for determining the levels of IL-4 in serum sample. |
| Biochem/physiol Actions: | IL-4 (interleukin 4) is a pleiotropic cytokine. It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior. |
| General description: | The Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysate and tissue lysate. |
| Immunogen: | Recombinant Mouse IL-4 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); 2X Cell Lysis Buffer (Item J); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |