Mouse IL-6 ELISA Kit
SIGMA/RAB0309 - for cell and tissue lysates
Synonym: B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; IFN-beta-2; IL-6
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse IL-6 ELISA Kit, Cat. No. RAB0309. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse IL-6 ELISA Kit, Cat. No. RAB0309, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 0.82-600 pg/mL |
| detection method | colorimetric |
| input | sample type tissue lysate sample type cell lysate |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA)Mouse IL-6 ELISA Kit has been used to measure the concentration of interleukin-6 (IL-6) in cell culture supernatants. |
| Biochem/physiol Actions: | IL-6 (interleukin 6) is a pleiotropic cytokine that mediates immune and inflammatory responses. It synergizes with IL-3 in megakaryocyte development and platelet production. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease and plasmacytoma/multiple myeloma. |
| General description: | The Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-6 cell lysate and tissue lysate. IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. |
| Immunogen: | Recombinant Mouse IL-6 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); 2X Cell Lysis Buffer (Item J); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |