Rat Interleukin-6 ELISA Kit
SIGMA/RAB0312 - for for cell lysate and tissue lysate
Synonym: IL-6; Interleukin-6 ; IL-6
Product Type: Chemical
Standard Curve : Typical Standard Curve for Rat Interleukin-6 (IL-6) ELISA Kit, Cat. No. RAB0312. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Rat Interleukin-6 (IL-6) ELISA Kit, Cat. No. RAB0312, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 40.96-10000 pg/mL |
| detection method | colorimetric |
| input | sample type tissue lysate sample type cell lysate |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | rat |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Rat Interleukin-6 ELISA Kit has been used to determine the plasma level of interleukin-6 (IL-6) via ELISA. |
| Biochem/physiol Actions: | Interleukin-6 (IL-6) acts as a neuromodulation factor. IL-6 has two signalling pathways, known as classic signalling pathway and trans-signalling pathway. It has a neuroprotective role in ischemic stroke, by activating IL-6/interleukin 6 receptor (IL-6R)/signal transducers and activators of transcription 3 (STAT3) signalling pathway. IL-6 is a multifunctional cytokine, which is implicated in acute inflammation. IL-6 is associated with SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome. |
| General description: | The Rat IL-6 ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-6 cell lysate and tissue lysate. |
| Immunogen: | Recombinant Rat IL-6 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); 2X Cell Lysis Buffer (Item J); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |