Human PSA-total ELISA Kit
SIGMA/RAB0331 - for serum, plasma, cell culture supernatant and urine
Synonym: Gamma-seminoprotein); Kallikrein-3; Prostate-specific antigen
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human PSA-total ELISA Kit, Cat. No. RAB0331. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human PSA-total ELISA Kit, Cat. No. RAB0331, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 10.24-2500 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type urine sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Human PSA-total ELISA Kit has been used to determine PSA (prostate specific antigen) level in serum of prostate cancer patients and cancer-free patients. |
| Biochem/physiol Actions: | Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA. |
| General description: | The Human PSA-total (Prostate Specific Antigen, total) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human PSA-total in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human PSA |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |