Human LIF ELISA Kit
SIGMA/RAB0339 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human LIF ELISA Kit, Cat. No. RAB0339. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human LIF ELISA Kit, Cat. No. RAB0339, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 8.23-6000 pg/mL |
| detection method | colorimetric |
| input | sample type urine sample type serum sample type cell culture supernatant(s) sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| General description: | Human leukemia inhibitory factor (LIF) Enzyme-Linked Immunosorbent Assay (ELISA) kit is an in vitro assay for the quantitative measurement of human LIF in biological samples, such as serum, plasma, cell culture supernatants, urine, and/or cell and tissue lysates. LIF protein is a monomeric glycoprotein and a member of the interleukin-6 (IL-6) family of cytokines. This protein acts in an autocrine and paracrine manner. LIF participates in several biological processes like an inflammatory response, embryonic implantation, cancer progression, differentiation of leukemia cell, neuronal development, and stem cell self-renewal. |
| Immunogen: | Recombinant Human LIF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |