Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines
SIGMA/RAB0352 - 192 assays
Product Type: Chemical
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable | |
| usage | 192 assays |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA) |
| General description: | The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. |
| Immunogen: | ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides |
| Symbol | ![]() ![]() GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H290 - H302 + H332 - H315 - H317 - H318 - H335 - H341 - H350 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-40-41-43 |
| Safety Statements | 26-36/37/39 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | 5x Blocking Solution (Item F); Fixing Solution (Item D); HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1); HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1); HRP-conjugated Anti-Mouse IgG Concentrate (Item I2); HRP-conjugated Anti-Mouse IgG Concentrate (Item I2); Uncoated Microplate; Quenching Solution for Cell-based ELISA Assay (Item E); Stop Solution; TMB Substrate Reagent (Item J); 20X Wash Buffer Concentrate A; 20X Wash Buffer Concentrate B |

(sandwich, competitive & Indirect ELISA)

