Human MMP-2 ELISA Kit
SIGMA/RAB0365 - for serum, plasma, cell culture supernatant
Synonym: MMP-2; Matrix metalloproteinase-2
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human MMP-2 ELISA Kit, Cat. No. RAB0365. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human MMP-2 ELISA Kit, Cat. No. RAB0365, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 3,500 pg/mL standard curve range: 3.3-800 ng/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type plasma sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA)Human MMP-2 ELISA kit has been used for the determination of MMP-2 in serum samples and cell supernatant. |
| Application: | Human MMP-2 ELISA Kit has been used in ELISA (enzyme-linked immunosorbent assay). |
| Biochem/physiol Actions: | Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, which has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. High levels of MMP-2 are observed in the acute phase of transverse myelitis. It is upregulated in colorectal carcinoma and is associated with poor survival outcome. MMP-2 gene haplotypes are also linked with risk of thoracic aortic dissection, a cardiovascular disorder with high death rate. |
| General description: | The Human MMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-2 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human MMP2 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer E (Item E2); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |