Human MMP-9 ELISA Kit
SIGMA/RAB0372 - for serum, plasma, cell culture supernatant and urine
Synonym: MMP-9; Matrix metalloproteinase-9
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human MMP-9 ELISA Kit, Cat. No. RAB0372. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human MMP-9 ELISA Kit, Cat. No. RAB0372, showing dilution factor.
| detection method | colorimetric |
| input | sample type serum sample type cell culture supernatant(s) sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Matrix metalloproteinase 9 (MMP9) is a gelatinase enzyme, which catalyzes the degradation of gelatin and collagen and is preferentially inhibited by tissue inhibitor of metalloproteinases 1 (TIMP-1). The MMP9 protein is a zinc-dependent protease which is responsible for degradation of extracellular matrix components. It also participates in the pathogenesis of cancers. MMP9 is associated with various disorders, such as cystic fibrosis, acute lung injury, asthma as well as respiratory syncytial virus infection both in vitro and in vivo. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis and multiple sclerosis and be regarded as a potential therapeutic target. Mutation of MMP-9 is associated with lumbar-disc herniation (LDH) and metaphyseal anadysplasia (MAD). |
| General description: | The Human MMP-9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-9 pro and active forms in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human MMP9 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer E (Item E2); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |