Human β-NGF ELISA Kit
SIGMA/RAB0380 - for serum, plasma, cell culture supernatant and urine
Synonym: Beta-NGF; Beta-nerve growth factor
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human β-NGF ELISA Kit, Cat. No. RAB0380. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human β-NGF ELISA Kit, Cat. No. RAB0380, showing dilution factor.
| detection method | colorimetric |
| input | sample type plasma sample type cell culture supernatant(s) sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Human β-NGF (nerve growth factor) ELISA (Enzyme linked immunosorbent assay) Kit has been used for the quantification of nerve growth factor in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (referred as CMS) , in all collected medium culture wastes. It has also been used for the quantification of nerve growth factor in lysate of decellularized meningeal scaffolds (DMS). |
| Biochem/physiol Actions: | NGFβ (nerve growth factor β ) is a potent neurotrophic factor that plays a vital role in development and maintenance of nervous systems and is essential for the differentiation and survival of neurons. It also mediates angiogenesis and facilitates progression and development of various cancer including, oral, breast and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB) and might also aid in determining the therapy for the same. It might alter placental function, as NGF signaling in placenta is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment. |
| General description: | The Human β-NGF (β-nerve growth factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human β-NGF in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human BNGF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer E (Item E2); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |