Human Platelet Factor-4 / CXCL4 ELISA Kit
SIGMA/RAB0402 - for serum, plasma, cell culture supernatant and urine
Synonym: C-X-C motif chemokine 4; Oncostatin-A; PF-4; Platelet factor 4; PF4
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Platelet Factor-4 / CXCL4 ELISA Kit, Cat. No. RAB0402. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Platelet Factor-4 / CXCL4 ELISA Kit, Cat. No. RAB0402, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 20 pg/mL standard curve range: 20.58-15000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type plasma sample type urine sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Human Platelet Factor-4 (PF4)/ CXCL4 (chemokine (C-X-C motif) ligand 4) ELISA Kit has been used to measure PF-4 / CXCL4 serum level in chronic myelomonocytic leukemia (CMML) patients. |
| Application: | Human Platelet Factor-4 / CXCL4 ELISA Kit has also been used in the measurement of platelet factor-4 in serum samples of prostate cancer and pseudoxanthoma elasticum patients. |
| Biochem/physiol Actions: | Platelet factor-4 (PF-4) or chemokine (C-X-C motif) ligand 4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. This protein associates with heparin and neutralizes it. It also neutralizes various other negatively charged proteoglycans. PF-4 / CXCL4 is implicated in cancer development. PF-4 regulates the activity of proangiogenic growth factors. PF-4 / CXCL4 function as a biomarker for systemic sclerosis and early tumor growth. PF-4 / CXCL4 is associated with various pathological conditions such as atherosclerosis, liver fibrosis and inflammation. |
| General description: | The Human PF-4 (Platelet Factor-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PF-4 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human PF4 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 1X Assay/Sample Diluent Buffer C (Item L); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |