Mouse Platelet Factor-4 / CXCL4 ELISA Kit
SIGMA/RAB0403 - for serum, plasma and cell culture supernatant
Synonym: C-X-C motif chemokine 4; PF-4; Platelet factor 4; cxcl4; PF4
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse Platelet Factor-4 / CXCL4 ELISA Kit, Cat. No. RAB0403. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse Platelet Factor-4 / CXCL4 ELISA Kit, Cat. No. RAB0403, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 6 pg/mL standard curve range: 4.12-3000 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| General description: | Platelet factor 4 (PF4/CXCL4), a plasma-circulating exosomal signature protein or chemokine packed in platelet α-granules, is liberated upon stimulation during inflammation and wound healing. It may participate in lymphedema. PF4 is considered a biomarker for lymphatic-promoted disorders. It can prevent angiogenesis. It can promote innate immune responses. In prothrombotic diseases, PF4 coupled to surface glycosaminoglycans on platelets, monocytes, and endothelial cells is also an immunogenic target. The mouse PF-4 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PF-4 in serum, plasma, cell culture supernatants, and urine. |
| Immunogen: | Recombinant Mouse PF4 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |