Human PAI-1 ELISA Kit
SIGMA/RAB0429 - for serum, plasma, cell culture supernatant.
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human PAI-I ELISA Kit, Cat. No. RAB0429. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human PAI-I ELISA Kit, Cat. No. RAB0429, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 35 pg/mL standard curve range: 0.102-25 ng/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type serum sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| General description: | The Human PAI-1 (plasminogen activator inhibitor-1) ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PAI-1 in serum, plasma, cell culture supernatants. PAI-1 is 45kDa serine proteinase inhibitor. It inhibits plasmogen activator and affects the fibrinolytic process, as a result of which it leads to thrombosis. Reduced fibrinolysis is observed in ischemic heart disease. While elevated PAI-1 levels are noticed in myocardial infarction. PAI-1 is associated with various physiological processes including wound healing, sepsis, insulin resistance, obesity, atherosclerosis, tumor angiogenesis, bone remodeling, asthma, rheumatoid arthritis, glomerulonephritis and chronic stress. |
| Immunogen: | Recombinant Human PAI-I |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |