Human ShhN ELISA Kit
SIGMA/RAB0430 - for serum, plasma, cell culture supernatants and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human ShhN ELISA Kit, Cat. No. RAB0430. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human ShhN ELISA Kit, Cat. No. RAB0430, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 42 pg/mL standard curve range: 40.96-10000 pg/mL (Assay Diluent C) |
| inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 40.96-10000 pg/mL (Assay Diluent B) |
|
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type serum sample type urine sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor patched (Ptc-1), de-represses smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar. |
| General description: | The Human Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human Shh-N in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human ShhN |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 1X Assay/Sample Diluent Buffer C (Item L); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |