Mouse Shh-N ELISA Kit
SIGMA/RAB0431 - for serum, plasma and cell culture supernatant
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse Shh-N ELISA Kit, Cat. No. RAB0431. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse Shh-N ELISA Kit, Cat. No. RAB0431, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 5 pg/mL standard curve range: 4.1-1000 pg/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type serum sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Mouse Shh-N ELISA Kit has been used to measure serum sonic hedgehog (Shh) level. |
| Biochem/physiol Actions: | Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar. |
| General description: | The Mouse Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Shh-N in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Mouse Sonic Hedgehog (N-terminus) |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |