Cell-Based ELISA for detecting phospho-STAT3 (pTyr705) in cultured cell lines
SIGMA/RAB0444 - 96 assays
Product Type: Chemical
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| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable | |
| usage | 96 assays |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Signal transducer and activator of transcription (STAT) proteins are a family of latent cytoplasmic transcription factors involved in cytokine, hormone, and growth factor signal transduction. STAT proteins mediate broadly diverse biological processes, including cell growth, differentiation, apoptosis, fetal development, transformation, inflammation, and immune response. Unlike all other members of the STAT gene family, ablation of STAT3 leads to embryonic lethality and it has been suggested that this protein might represent a primordial STAT protein. It evokes a number of distinct responses in different cells, including induction of an acute-phase response in hepatoma cells, stimulation of proliferation in B lymphocytes, activation of terminal differentiation and growth arrest in monocytes, and maintenance of the pluripotency of embryonic stem cells. Dysregulation of STAT3 signaling has been demonstrated to contribute to malignant cellular transformation. Src kinase-mediated activation of STAT3 has been shown to be essential in prostate and ovarian carcinomas. In head and neck cancers, constitutive STAT3 activity with up-regulated epidermal growth factor receptor (EGFR) signaling plays an important role in malignant proliferation. STAT3 may be a key player in the pathogenesis of diverse human cancers which makes this molecule a prime target for novel therapies. |
| General description: | The Cell-Based Stat3 (pTyr705) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat3 (pTyr705) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. The STAT3 (signal transducer and activator of transcription)gene is mapped to human chromosome 17q21.2. Upon phosphorylation, STAT3 translocates to nucleus from cytoplasm, where it mediates a number of biological processes. The protein structure includes the following domains : N-domain, coiled-coil domain, DNA-binding domain, linker domain, SH2 domain and transcriptional activation domain. |
| Immunogen: | Stat3 (pTyr705) synthetic phosphopeptide |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-40-41-43 |
| Safety Statements | 26-36/37/39 |
| RIDADR | UN 3264 8 / PGIII |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | 5x Blocking Solution (Item F); Fixing Solution (Item D); HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1); HRP-conjugated Anti-Mouse IgG Concentrate (Item I2); Uncoated Microplate; Quenching Solution for Cell-based ELISA Assay (Item E); Stop Solution; TMB Substrate Reagent (Item J); 20X Wash Buffer Concentrate A; 20X Wash Buffer Concentrate B |