Human Thyroglobulin ELISA Kit
SIGMA/RAB0458 - for serum, plasma, cell culture supernatants and urine
Synonym: TGF-beta-1; Thyroglobulin
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Thyroglobulin ELISA Kit, Cat. No. RAB0458. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Thyroglobulin ELISA Kit, Cat. No. RAB0458, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 220 pg/mL standard curve range: 0.205-50 ng/mL |
| detection method | colorimetric |
| input | sample type urine sample type plasma sample type cell culture supernatant(s) sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Human Thyroglobulin ELISA Kit has been used to measure the secreted thyroglobulin (TG) level in primary human thyrocytes. |
| Biochem/physiol Actions: | Thyroglobulin (TG) is a prothyroid hormone. It plays a vital role in production of L-thyroxine (tetraiodothyronine, T4) and L-triiodothyronine (T3) hormones in thyroid gland. Tyrosyls of TG acts as substrate for iodination and hormone formation. Serum thyroglobulin level is used as marker of metastases in differentiated thyroid carcinomas. Mutation in the gene is associated with the development of congenital goitrous hypothyroidism and non-endemic simple goitre. |
| General description: | The Human Thyroglobulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Thyroglobulin in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human Thyroglobulin |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 1X Assay/Sample Diluent Buffer C (Item L); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |