Human TIMP-2 ELISA Kit
SIGMA/RAB0472 - for serum, plasma, cell culture supernatant and urine
Synonym: Metalloproteinase inhibitor 2 ; TIMP-2; Tissue inhibitor of metalloproteinases 2
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human TIMP-2 ELISA Kit, Cat. No. RAB0472. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human TIMP-2 ELISA Kit, Cat. No. RAB0472, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.6-1200 pg/mL |
| detection method | colorimetric |
| input | sample type urine sample type cell culture supernatant(s) sample type serum sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Human TIMP-2 ELISA Kit has been used to measure tissue inhibitor of metalloproteinase-2 (TIMP-2) level in blood samples from Atherosclerotic renal artery stenosis (ARAS) patients. |
| Biochem/physiol Actions: | Tissue inhibitor of metalloproteinase-2 (TIMP-2) plays a vital role in inhibition of matrix metalloproteinases (MMPs) and functions as a key modulator of extracellular matrix deposition and degradation in various physiological processes. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. TIMP-2 mediates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22. TIMP-2 functions as a biomarker for cell cycle arrest. |
| General description: | The Human TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIMP-2 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human TIMP2 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |