Mouse Tumor Necrosis Factor α ELISA Kit
SIGMA/RAB0477 - for serum, plasma and cell culture supernatant
Synonym: Cachectin; TNF-alpha; Tumor necrosis factor alpha; TNF-alpha
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse Tumor Necrosis Factor-α ELISA Kit, Cat. No. RAB0477. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse Tumor Necrosis Factor-α ELISA Kit, Cat. No. RAB0477, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 60 pg/mL standard curve range: 93.75-6000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type cell culture supernatant(s) sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples. |
| Biochem/physiol Actions: | Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2. |
| General description: | The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants. |
| General description: | Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α. |
| Immunogen: | Recombinant Mouse TNF-α |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| Precautionary statements | P234 - P390 |
| RIDADR | UN 3264 8 / PGIII |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |