Rat Tumor Necrosis Factor α ELISA Kit
SIGMA/RAB0480 - for cell and tissue lysates
Synonym: Cachectin; TNF-alpha; Tumor necrosis factor alpha; TNF-alpha
Product Type: Chemical
Standard Curve : Typical Standard Curve for Rat Tumor Necrosis Factor-α ELISA Kit, Cat. No. RAB0480. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Rat Tumor Necrosis Factor-α ELISA Kit, Cat. No. RAB0480, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 25 pg/mL standard curve range: 82.3-20000 pg/mL |
| detection method | colorimetric |
| input | sample type cell lysate sample type tissue lysate |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | rat |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate. |
| Biochem/physiol Actions: | Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer’s disease, multiple sclerosis, Parkinson’s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats. |
| General description: | The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate. |
| Immunogen: | Recombinant Rat TNF-α |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); 2X Cell Lysis Buffer (Item J); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |