Human p53 ELISA Kit
SIGMA/RAB0500 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human p53 ELISA Kit, Cat. No. RAB0500. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 0.3 ng/mL standard curve range: 0.328-80 ng/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type urine sample type serum sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells. |
| General description: | Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human p53 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 5X Assay/Sample Diluent Buffer D (Item K); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |