Human VEGF ELISA Kit
SIGMA/RAB0508 - for cell and tissue lysates
Synonym: VEGF-A; Vascular endothelial growth factor A ; VEGF-A
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human VEGF ELISA Kit, Cat. No. RAB0508. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 8.23-6000 pg/mL |
| detection method | colorimetric |
| input | sample type cell lysate sample type tissue lysate |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves’ disease, psoriasis, cardiovascular disease, and peripheral limb disease. |
| General description: | The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate. |
| Immunogen: | Recombinant Human VEGF-A |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Sample Diluent Buffer (Item D2); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); 2X Cell Lysis Buffer (Item J); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |