Human IL18 / Interleukin-18 ELISA Kit
SIGMA/RAB0543 - for serum, plasma, cell culture supernatants and urine
Synonym: IFN-gamma-inducing factor; IL-18; Interleukin-18
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human IL18 / Interleukin-18 ELISA Kit, Cat. No. RAB0543. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
| assay range | inter-assay cv: <10% intra-assay cv: <12% sensitivity: 0.5 pg/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type urine sample type plasma sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Application: | Human IL18 / Interleukin-18 ELISA Kit has been used to evaluate the macrophage cytokines production in response to trans-stilbene and terphenyl compounds. It has also been used to analyze and measure IL-18 levels in the urine of chronic kidney disease patients. |
| General description: | The antibody pair provided in this kit detects Human IL-18 in serum, plasma, cell culture supernatants, and urine. Interleukin-18 (IL-18) is a proinflammatory cytokine, expressed in most of the mammalian tissues including adipose, liver and skeletal muscle. It is generated as pro-IL-18 which undergoes cleavage to form a mature IL-18 possessing the signal peptide. IL-18 induces Th1 (T helper cell 1) lymphocyte action mediated by interferon-γ. It controls the metabolism of lipids and glucose. Inflammasome activation is responsible for IL-18 release, which is a significant event in response to viral infection. Increased level of IL-18 is observed in kidney and liver disease, diabetes, atherosclerosis, autoimmune disease such as rheumatoid arthritis and asthma. It is also associated with the development of renal dysfunction. |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); ELISA Stop Solution (Item I); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 1X Assay/Sample Diluent Buffer A (Item D1); 20X Wash Buffer (Item B) |