Mouse Tnfsf4 / Tumor Necrosis Factor Ligand Superfamily Member 4 ELISA Kit
SIGMA/RAB0564 - for serum, plasma and cell culture supernatants
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse Tnfsf4 / Tumor Necrosis Factor Ligand Superfamily Member 4 ELISA Kit, Cat. No. RAB0564. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
| assay range | inter-assay cv: <10% intra-assay cv: <12% sensitivity: 30 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Tumor necrosis factor (TNF) superfamily member 4 (Tnfsf4) signals specifically through the OX40 receptor, which is expressed primarily on CD4+T cells but also on certain activated CD8+T cells. Tnfsf4 acts as a costimulatory signal, which is essential for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival and increases expression of regulated on activation, normal T cell expressed and secreted (RANTES) or chemokine ligand 5 (CCL5), interleukins-2 and -3 and interferon γ (IFNg). Tnfsf4 signaling plays an important role in immuno-regulatory communication and immune-tolerance. Polymorphisms in the TNFSF4 gene leads to the development of autoimmune thyroid diseases (AITDs), rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). |
| General description: | This ELISA antibody pair detects mouse TNFSF4/OX40. |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); ELISA Stop Solution (Item I); ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); 20X Wash Buffer (Item B) |