Human AFP / Alpha-fetoprotein ELISA Kit
SIGMA/RAB0602 - for serum, plasma, cell culture supernatants and urine
Product Type: Chemical
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| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 6 pg/mL standard curve range: 7.373-1800 pg/mL |
| detection method | colorimetric |
| input | sample type urine sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| Application: | Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA)Human α-fetoprotein (AFP) ELISA kit has been used to detect levels of AFP in comparison with photoelectrochemical (PEC) immunosensor. |
| Biochem/physiol Actions: | α-fetoprotein (AFP) facilitates the regulation of fatty acids in both fetal and proliferating adult liver cells. Overexpression of the gene has been observed in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It functions as a tumor marker for HCC (hepatocellular carcinoma). |
| General description: | The AFP ELISA kit provides for the quantitative measurement of Alpha-fetoprotein in Cell Culture Supernatants, Plasma and Serum. |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); ELISA Stop Solution (Item I); ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); 20X Wash Buffer (Item B) |