Human C5a / Complement C5a ELISA Kit
SIGMA/RAB0631 - for serum, plasma, and cell culture supernatants
Synonym: Complement C5a
Product Type: Chemical
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 31 pg/mL standard curve range: 31.25-2000 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type cell culture supernatant(s) sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA) |
| General description: | Human complement 5a (C5a) is generated during complement activation from the α-chain of complement C5. It binds to a signaling G-protein coupled receptor (GPCR), inducing neutrophil chemotaxis and endothelial cell activation. C5a also triggers an oxidative burst in macrophages and neutrophils and induces release of histamine in basophils and mast cells. |
| General description: | The antibody pair provided in this kit recognizes human Complement Component C5a. |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol | GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); ELISA Stop Solution (Item I); ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); 20X Wash Buffer (Item B) |

(sandwich, competitive & Indirect ELISA)