Mouse Ins1 / Insulin-1 ELISA Kit
SIGMA/RAB0817 - for serum, plasma and cell culture supernatants
Synonym: Insulin
Product Type: Chemical
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| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 5 μIU/mL standard curve range: 6.25-400 μIU/ml |
| detection method | colorimetric |
| input | sample type plasma sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| Application: | Mouse Ins1 / Insulin-1 ELISA Kit has been used to measure changes in serum insulin in male Sprague-Dawley rats with chemically induced diabetes. For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region. The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Knockout of Ins-1 and Ins-2 leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth. |
| General description: | The antibody pair provided in this kit recognizes Mouse Insulin 1. |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); ELISA Stop Solution (Item I); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 1X Assay/Sample Diluent Buffer C (Item L); 20X Wash Buffer (Item B) |