HEPES
SIGMA/RDD002 - anhydrous, free-flowing, Redi-Dri™, ≥99.5%
Synonym: 4-
CAS Number: 7365-45-9
Empirical Formula (Hill Notation): C8H18N2O4S
Molecular Weight: 238.30
EC Number: 230-907-9
MDL Number: MFCD00006158
Linear Formula: C8H18N2O4S
Product Type: Chemical
Loss on Drying Graph
Free-flowing salts
The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: RDD002-100G
This image depicts SKU: RDD002-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: RDD002-1KG
This image depicts SKU: RDD002-1KG
| assay | ≥99.5% |
| form | crystalline powder |
| grade | anhydrous |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| pH | 5.0-6.5 (25 °C, 238 g/L) |
| pKa (25 °C) | 7.5 |
| product line | Redi-Dri™ |
| quality | free-flowing |
| Quality Level | 100  |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| solubility | water: 500 mg/mL, clear, colorless |
| useful pH range | 6.8-8.2 |
| Application: | HEPES has been used: • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions • As a component in intracellular solution for the preparation of brain-slice recording • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification • To maintain the pH at 7 in thermal denaturation assay of purified protein • In isocratic elution of protein using gel filtration • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy |
| General description: | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| Legal Information: | Redi-Dri is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 2.5 kg in poly drum |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% |
| UNSPSC | 12161700 |