Trizma® base
SIGMA/RDD008 - anhydrous, free-flowing, Redi-Dri™, ≥99.9%
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
Loss on Drying Graph
Free-flowing salts
The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: RDD008-100G
This image depicts SKU: RDD008-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: RDD008-1KG
This image depicts SKU: RDD008-1KG
| λ | (A290 UV absorbance < 0.05) |
| in 5 mM Tris; DNA (40% (w/w)) | |
| absorption | ≤0.05 at 290 nm at 40% |
| assay | ≥99.9% |
| bp | 219-220 °C/10 mmHg (lit.) |
| color | white |
| description | aminopeptidase substrate |
| does not contain | Carbon Fiber |
| form | powder |
| grade | anhydrous |
| impurities | ≤0.2% Water (Karl Fischer) |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| mp | 167-172 °C (lit.) |
| pH | 10.5-12 |
| 7-9 | |
| pKa (25 °C) | 8.1 |
| product line | Redi-Dri™ |
| quality | free-flowing |
| Quality Level | 100  |
| SMILES string | NC(CO)(CO)CO |
| solubility | water: soluble (Water < 0.2%) |
| useful pH range | 7-9 |
| Application: | Tris base has been used: • To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization • In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting • In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein • As a component of nuclei extraction buffer |
| General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
| Legal Information: | Redi-Dri is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 2.5 kg in poly drum |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.9% |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| UNSPSC | 12161700 |