ReadyScript® cDNA Synthesis Mix
SIGMA/RDRT - Complete reagent for first strand cDNA synthesis for RT-qPCR
Synonym: cDNA Synthesis Mix
Product Type: Chemical
Representative kit contents
A sensitive and easy-to-use reagent for two-step RT-qPCR
| color | colorless |
| feature | dNTPs included |
| hotstart: no | |
| input | purified RNA |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | RT-PCR: suitable |
| usage | sufficient for 100 reactions |
| sufficient for 25 reactions | |
| sufficient for 500 reactions |
| Application: | ReadyScript® cDNA Synthesis Mix has been used for complementary DNA (cDNA) synthesis from total/mRNA in 2-step reverse transcription polymerase chain reaction (RT-PCR) and quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays for gene expression studies. |
| Features and Benefits: | • Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template • Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase • Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes • Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up • Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month |
| Features and Benefits: | • Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product • High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components • RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification • Reverse transcribes both rare and abundant genes with a wide input range of template • Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template |
| General description: | ReadyScript® cDNA Synthesis Mix is a sensitive and easy-to-use solution for two-step RT-PCR. This 5X concentrated mix provides all necessary components (except RNA template) for first-strand synthesis including: buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, ReadyScript reverse transcriptase and stabilizers. ReadyScript is a RNase H(+) derivative of MMLV reverse transcriptase, optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The unique blend of oligo (dT) and random primers in the ReadyScript cDNA Synthesis Mix works exceptionally well with a wide variety of targets. It is optimized for the production of targets < 1kb in length. ReadyScript cDNA Synthesis Mix produces excellent results in both real-time and conventional RT-PCR. |
| Legal Information: | ReadyScript is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Stable for 1 year when stored in a constant temperature freezer at -20 °C. To extend product′s shelf-life, store the kit at -70 °C. Readyscript cDNA Synthesis Mix showed no loss in functional performance after 20 cycles of freezing on dry ice and thawing on ice. However, we recommend keeping the freeze-thaw cycles to a minimum. |
| Other Notes: | Supplied as a 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), recombinant RNase inhibitor protein, ReadyScript reverse transcriptase, random primers, oligo(dT) primer and stabilizers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |