GenElute™ Plasma/Serum RNA Purification Maxi Kit
SIGMA/RNB700
Product Type: Product-on-demand
GenElute™ Plasma/Serum RNA Purification Maxi Kit, Cat. No. RNB700
Purification of cell-free circulating RNA and exosomal RNA from different plasma volumes. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was used to purify cell-free circulating and exosomal RNA from 1.5 mL, 3 mL and 5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two millilitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of the (A) housekeeping 5S rRNA transcript and (B) miR-21. The average Ct. value for both the 5S rRNA transcript and the miR-21 is linearly decreasing with increasing the sample input volume.
Purification of cell-free circulating RNA and exosomal RNA from different plasma volumes. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was used to purify cell-free circulating and exosomal RNA from 1.5 mL, 3 mL and 5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two millilitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of the (A) housekeeping 5S rRNA transcript and (B) miR-21. The average Ct. value for both the 5S rRNA transcript and the miR-21 is linearly decreasing with increasing the sample input volume.
GenElute™ Plasma/Serum RNA Purification Maxi Kit, Cat. No. RNB700
Linearity of RNA purified from increasing plasma volumes using Sigma’s Plasma/Serum RNA Purification Maxi Kit. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was used to purify RNA from 1.5 mL, 3 mL and 5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of the housekeeping (A) 5S rRNA transcript and (B) miR-21 from the different plasma volumes. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was able to recover 92% of the 5S rRNA transcript from 3 mL plasma relative to the amount that is present in 1.5 mL plasma. Moreover, 90% of the 5S rRNA transcript was recovered from 5 mL plasma relative to the amount that is present in 3 mL plasma. As for miR-21, Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was able to recover 91% of miR-21 from 3 mL plasma relative to the amount that is present in 1.5 mL plasma. Furthermore, 90% of miR-21 was recovered from 5 mL plasma relative to the amount that is present in 3 mL plasma.
Linearity of RNA purified from increasing plasma volumes using Sigma’s Plasma/Serum RNA Purification Maxi Kit. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was used to purify RNA from 1.5 mL, 3 mL and 5 mL plasma prepared from blood collected on citrate as an anticoagulant. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of the housekeeping (A) 5S rRNA transcript and (B) miR-21 from the different plasma volumes. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was able to recover 92% of the 5S rRNA transcript from 3 mL plasma relative to the amount that is present in 1.5 mL plasma. Moreover, 90% of the 5S rRNA transcript was recovered from 5 mL plasma relative to the amount that is present in 3 mL plasma. As for miR-21, Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit was able to recover 91% of miR-21 from 3 mL plasma relative to the amount that is present in 1.5 mL plasma. Furthermore, 90% of miR-21 was recovered from 5 mL plasma relative to the amount that is present in 3 mL plasma.
GenElute™ Plasma/Serum RNA Purification Maxi Kit, Cat. No. RNB700
Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and miR-21. DNA was isolated from 1.5 mL, 3 mL and 5 mL plasma using Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit. Increasing volumes of the elution (2, 4 and 8 μL) were used in a 20 μL reverse transcription reaction followed by qPCR amplification reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in the PCR input volume used as a template in the reverse transcription reaction did not affect the Ct value generated from the qPCR amplification for both (A) 5S rRNA transcript and (B) miR-21. In fact the Ct. values tend to decrease with increasing the PCR input volume indicating that RNA purified from plasma using Sigma’s kit is free of the common inhibitors usually present in plasma.
Determination of the amount of inhibition present in plasma RNA samples when detecting the human 5S transcript and miR-21. DNA was isolated from 1.5 mL, 3 mL and 5 mL plasma using Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit. Increasing volumes of the elution (2, 4 and 8 μL) were used in a 20 μL reverse transcription reaction followed by qPCR amplification reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in the PCR input volume used as a template in the reverse transcription reaction did not affect the Ct value generated from the qPCR amplification for both (A) 5S rRNA transcript and (B) miR-21. In fact the Ct. values tend to decrease with increasing the PCR input volume indicating that RNA purified from plasma using Sigma’s kit is free of the common inhibitors usually present in plasma.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| shipped in | ambient |
| storage temp. | room temp |
| sustainability | Greener Alternative Product |
| Application: | • Reverse transcription qPCR • Reverse transcription PCR • Northern blotting • RNase protection and primer extension • Expression array assays • miRNA array • RNA-Seq • Nanostring • Fluidigm • Droplet/Digital PCR |
| General description: | Click here to view the kit protocol |
| General description: | Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 2 mL and up to 5 mL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma’s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Sigma’s GenElute™ Plasma/Serum RNA Purification Maxi Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual. Normally, the RNA yield from plasma or serum RNA is highly variable (ranging from 1 to 100 ng/mL). • Isolate all sizes of circulating and exosomal RNA, including microRNA • Versatile plasma/serum input ranging from 2 mL to 5 mL • No phenol extractions • No carrier RNA • Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 50 μL to 100 μL • Purify high-quality RNA in 35-40 minutes |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Preps |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | room temp |
| UNSPSC | 41105600 |