Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels
SIGMA/RPROB
Product Type: Chemical
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| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | protein staining: suitable |
| usage | sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels) |
| Application: | R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide. |
| Other Notes: | Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Solution A 500 mL; Solution B 500 mL |