GenElute™ 96 Well Total RNA Purification Kit
SIGMA/RTN9604 - 4 x 96 well plate, sufficient for 4 96-well plate purifications
Synonym: High Throughput Purification, RNA Purification
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| quality | 4 x 96 well plate |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | RNA purification: suitable |
| usage | sufficient for 4 96-well plate purifications |
| Application: | The purified total RNA is ready for use in any enzymatic downstream application. |
| Biochem/physiol Actions: | Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit. |
| Features and Benefits: | • Unique plate design offers low risk of cross-contamination • RNA can be used for any kind of downstream enzymatic reaction • Processing possible under with a vacuum or centrifuge • Suitable for manual and automated processing |
| General description: | Click here to view the kit protocol |
| General description: | GenElute 96 Well Total RNA Purification Kit allows for the high-throughput purification of total RNA from cells or tissue. The kit utilizes the advantages of silica membrane technology and eliminates the use of hazardous organic compounds such as phenol and chloroform. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Application notes: Method of purification: Silica-membrane technology Compatible with Manual or automated, vacuum or centrifugation Efficient recovery of > 200 nt Typical yield 20 μg (2 x 106 HeLa cells), 20 μg (20 mg mouse liver) Typical concentration 50–200 ng/μL Elution volume 50–130 μL Preparation time 70 min/plate Binding capacity 100 μg RNA ratio 28S/18S approx. 2.1 A260/A280 1.90–2.10 Typical RIN (RNA > 9 (cells) Sufficient for: < 107 cultured cells (centrifugation) < 30 mg tissue (centrifugation) saliva Related products: Prod # Related prod # RTN9604 RTN9602 RTN9602 RTN9604 |
| Packaging: | Components included:• Qty in kit: Part number: L7170 Component description: RNA Lysis Solution • Qty in kit: Part number: W3395 Component description: RNA Wash Buffer 1 • Qty in kit: Part number: W3290 Component description: RNA Wash Buffer 2, Concentrate • Qty in kit: Part number: W3645 Component description: RNA Wash Buffer 3, Concentrate • Qty in kit: Part number: R2658 Component description: Reaction Buffer for rDNase • Qty in kit: Part number: R2783 Component description: rDNase, Rnase-free • Qty in kit: Part number: R2908 Component description: Rnase-free Water • Qty in kit: Part number: R3033 Component description: RNA Binding Plates, blue ring • Qty in kit: Part number: T8330 Component description: Collection Tubes, 1.5mL • Qty in kit: Part number: W3270 Component description: Wash Plates • Qty in kit: Part number: S9326 Component description: Square-well Blocks • Qty in kit: Part number: E5911 Component description: Elution plate U-Bottom • Qty in kit: Part number: R3408 Component description: Round-well Blocks Low |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 + H332 - H314 - H317 - H334 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 20/21/22-32-42/43-52/53 |
| Safety Statements | 23-36/37-45-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Supplemental Hazard Statements | EUH032 |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | RNA Lysis Solution 125 mL; RNA Wash Buffer 1 360 mL; RNA Wash Buffer 2, Concentrate 2 x 90 mL; RNA Wash Buffer 3, Concentrate 2 x 54 mL; Reaction Buffer for rDNase 4 x 20 mL; rDNase, RNase-free 8 vial(s); RNase-free Water 2 x 54 mL; RNA Binding Plates, blue ring 4 ea; Collection Tubes, 1.5 mL 16 ea; Wash Plates 4 ea; Square-well Blocks 6 ea; Elution Plate, U-Bottom 4 ea; Round-well Blocks Low 4 ea |