Sonic Hedgehog Peptide from mouse
SIGMA/S0191 - ≥97% (SDS-PAGE), recombinant, expressed in E. coli, Fused to a histidine tag at the C-terminus, lyophilized powder
Synonym: Shh
MDL Number: MFCD03792594
Product Type: Chemical
SDS-PAGE Sonic Hedgehog Peptide from Mouse (Cat. No. S0191) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Sonic Hedgehog Peptide that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥97% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | <1 EU/μg |
| mol wt | predicted mol wt 20 kDa |
| packaging | pkg of 25 μg |
| potency | 0.4-3.0 μg/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | Q62226  |
| Application: | Sonic hedgehog peptide from mouse has been used for neural differentiation. |
| Biochem/physiol Actions: | Cell signaling peptide involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube. |
| Biochem/physiol Actions: | Involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube. |
| Biochem/physiol Actions: | Sonic hedgehog (Shh) has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. |
| General description: | Sonic hedgehog (Shh) is a morphogenic glycoprotein. The gene encoding it is localized on mouse chromosome 5. |
| Other Notes: | N-terminal peptide encoding amino acid residues 25-198. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose and 50 μg of bovine serum albumin per 1 μg of cytokine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |