Sonic Hedgehog Peptide from mouse

SIGMA/S0191 - ≥97% (SDS-PAGE), recombinant, expressed in E. coli, Fused to a histidine tag at the C-terminus, lyophilized powder

Synonym: Shh

MDL Number: MFCD03792594
Product Type: Chemical

SDS-PAGE Sonic Hedgehog Peptide from Mouse (Cat. No. S0191) was assessed on SDS-PAGE.

Bioassay EC₅₀ is defined as the effective concentration of Sonic Hedgehog Peptide that elicits a 50% attachment factor in cell based bioassay.

assay	≥97% (SDS-PAGE)
biological source	mouse
form	lyophilized powder
impurities	<1 EU/μg
mol wt	predicted mol wt 20 kDa
packaging	pkg of 25 μg
potency	0.4-3.0 μg/mL ED₅₀
Quality Level	200
recombinant	expressed in E. coli
storage condition	avoid repeated freeze/thaw cycles
storage temp.	−20°C
UniProt accession no.	Q62226

Application:	Sonic hedgehog peptide from mouse has been used for neural differentiation.
Biochem/physiol Actions:	Cell signaling peptide involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube.
Biochem/physiol Actions:	Involved in embryonic morphogenic development of several tissues including Hensen′s node, the notochord, and the neural tube.
Biochem/physiol Actions:	Sonic hedgehog (Shh) has a role in fracture healing and regeneration. The protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer.
General description:	Sonic hedgehog (Shh) is a morphogenic glycoprotein. The gene encoding it is localized on mouse chromosome 5.
Other Notes:	N-terminal peptide encoding amino acid residues 25-198.
Physical form:	Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose and 50 μg of bovine serum albumin per 1 μg of cytokine.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3